A-1821A: Choosing Which Optical System of the OptimaTM XL-I Analytical Ultracentrifuge to Use

DS-819: Overview of Sedimentation Velocity for the OptimaTM XL-A Analytical Ultracentrifuge

A-1815A: Rapid Determination of Spectrophotometric Absortivity by Analytical Ultracentrifugation

Spectrophotometric determination of protein concentrations is an important analytical tool for nearly all phases of protein drug development and manufacturing. Determinations of absorptivities (A0.1%) and molar extinction coefficients (ε) for protein solutions require at least one initial, accurate measurement of protein concentration. Traditionally, this crucial concentration has been determin...

DS-834: The Solution Conformation of Novel Antibody Fragments Studied Using the OptimaTM XL-A Analytical Ultracentrifuge

One of the latest attempts to improve monoclonal antibodies in their use as radiolabeled diagnostic markers (1) has involved the cross-linking of novel Fab' fragments. It is expected that a relationship exists between the spacing of the antigen binding sites at the extremes of the Fab' frag-ments in solution and their immunological performance in vitro and in vivo. In order to properly understa...

Performance of a fast fiber based UV/Vis multiwavelength detector for the analytical ultracentrifuge

The optical setup and the performance of a prototype UV/Vis multiwavelength analytical ultracentrifuge (MWL-AUC) is described and compared to the commercially available Optima XL-A from Beckman Coulter. Slight modifications have been made to the optical path of the MWL-AUC. With respect to wavelength accuracy and radial resolution, the new MWL-AUC is found to be comparable to the existing XL-A....

Rapid Plasmid Isolations Using Efficient Sedimentation Program Overspeed Control in␣ the OptimaTM XL Ultracentrifuge from Beckman

BECKMAN stressing. ESP allows rotor speed to be reduced automatically before precipitation can occur. Since the time required for gradient formation decreases with faster rotor speeds, this approach results in more rapid separations. In general, for fixed angle rotors, rotor speed is limited by the need to avoid precipitation of the gradient salt at the bottom of the tube, where the concentrati...